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Understanding the Types of Biological Safety Cabinet (BSC)

Updated: Mar 23, 2023

Biosafety cabinets are essential equipment in laboratory and pharmacy environments. They provide a safe working environment for laboratory personnel who handle hazardous biological materials, including pathogens and other potentially infectious materials. Biological safety cabinets come in different classes, each with unique features and benefits. In this blog post, we will explore the different classes of biological safety cabinets and their features and benefits.


Class I Biological Safety Cabinets

Class I biological safety cabinets provide personnel and environmental protection. They are designed to protect laboratory personnel from exposure to hazardous biological materials, such as infectious agents and toxins. They also protect the laboratory environment by preventing the release of contaminants into the air.

Features:

  • Exhaust air is filtered through a HEPA filter

  • The front opening is protected by a sliding sash or a hinged door

  • Airflow is maintained by a blower or fan

  • The cabinet is not suitable for working with volatile or toxic chemicals

Benefits:

  • Protects personnel from exposure to hazardous biological materials

  • Prevents the release of contaminants into the air

  • Relatively inexpensive compared to other classes of biological safety cabinets

Class II Biological Safety Cabinets

Class II biological safety cabinets are the most commonly used type of biological safety cabinet in laboratory and pharmacy environments. They provide personnel, environmental, and product protection. They are designed to provide a sterile working environment for laboratory personnel who handle hazardous biological materials.

Features:

  • Exhaust air is filtered through a HEPA filter

  • Airflow is maintained by a blower or fan

  • The front opening is protected by a sliding sash or a hinged door

  • The cabinet is divided into two areas, the work area and the exhaust area

  • The work area is maintained at a negative pressure, preventing the release of contaminants into the environment

Benefits:

  • Provides a sterile working environment for laboratory personnel

  • Protects personnel, the environment, and products from exposure to hazardous biological materials

  • Suitable for working with volatile or toxic chemicals

Class III Biological Safety Cabinets

Class III biological safety cabinets provide maximum protection to personnel, the environment, and the product. They are designed to handle the most hazardous biological materials, including those that require the highest level of containment.

Features:

  • Exhaust air is filtered through two HEPA filters

  • The cabinet is a completely enclosed, gas-tight unit

  • Personnel access the cabinet through attached gloves and an airlock

  • All materials are passed through a double-door autoclave before removal from the cabinet

Benefits:

  • Provides maximum protection to personnel, the environment, and the product

  • Suitable for handling the most hazardous biological materials, including those that require the highest level of containment

  • Allows for safe handling of infectious agents that pose a high risk of aerosol transmission




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